This research dealt with the issue of constitutional guarantees of human rights provided when applying the provisions of the Jordanian Penal Code. In this context, the research included human rights issues in the Jordanian constitution, human rights challenges in Jordan, in addition to human rights guarantees in the Jordanian constitution. The researcher used the descriptive approach to study the Jordanian legislation related to the issue of constitutional guarantees for human rights in the face of the application of the provisions of the Penal Code. The researcher concluded that the Jordanian legislator pays special attention to the issue of human rights, as it is evident in the second chapter of the Jordanian constitution, which is devoted to the rights and duties of Jordanians. However, the provisions of the Jordanian constitution did not explicitly and clearly state human rights guarantees when applying the provisions of the Jordanian Penal Code.