Nüshu is the only living female script in the world. It is a complete set of writing signs created and used by women in Jiangyong county and Daoxian County of Hunan Province. Since it was discovered by Chinese scholars in the 1980s, scholars in China and the worldwide have studied Nüshu in succession. However, there are few studies on Nüshu from the perspective of semiotics. Based on Peirce’s theories of semiotics and pragmaticism, this paper makes an exploratory analysis on the locutionary meaning, illocutionary meaning and perlocutionary meaning of Nüshu from the perspective of Morris’ pragmatics, expecting to play a role in the multi-path study of Nüshu signs.